牦牛Ihh基因组织表达分析、SNP检测及其基因型组合与生产性状的关联分析

doi:10.11843/j.issn.0366-6964.2015.01.007

Abstract

Abstract:

The objective of this study was to identify the polymorphisms of Ihh gene and their correlation with production traits，then to detect the molecular markers related to production traits，and investigate distribution and expression in tissues detected in yak and cattle.At the same time，RT-PCR method was used to detect the expression of Ihh gene in different tissues in yak and cattle.BLAST and Chromas were used to screen the SNPs in Ihh gene by the wave height of sequencing map.The genotypes were determined by high-resolution melting curve(HRM) and the alleles frequency was estimated.The PHASE and SHEsis softwares were used to analyze matching chain disequilibrium and haplotype，respectively.The SPSS17.0 software was used for association analysis.Two SNPs(5855(C/T) and 6383(G/A)) of Ihh gene were indentified in the population，which were on exon 3.Through population genetics analysis，the results showed that 2 loci were at high polymorphic status(PIC>0.25).The χ²tests showed that the 2 loci were in the status of Hardy-Weinberg equilibrium(P＜0.05) in Gannan and Datong yaks; the 2 loci were not all in the status of Hardy-Weinberg equilibrium(P＞0.05) in Tianzhu yak.Analysis of matching chain disequilibrium and haplotype showed that there was a strengh linkage equilibrium between the 2 loci with 4 haplotype combinations and 3 genotype combinations.Analysis of association of polymorphism with body measurement traits at all mutation loci showed that 3 genotype combinations had significant effects on body height，body length，weight，tube girth and herrt girth(P＜0.05).Yak with CTGA genotype combination had higher body height，body length，weight，tube girth and herrt girth than individuals with CCGG and TTAA.The result of expression showed that Ihh gene was expressed in 8 different tissues.The results suggest that Ihh gene may have potential effects on production traits in the above mentioned yak populations and could be used for marker-assisted selection.

CLC Number:

LI Tian-ke，ZHAO Juan-hua，PEI Jie，LIANG Chun-nian，GUO Xian，QIN Wen，YAN Ping. Tissue Expression，SNP Detection and Association of Genotype Combination of Ihh Gene with Production Traits in Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(1): 50-59.

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Tissue Expression，SNP Detection and Association of Genotype Combination of Ihh Gene with Production Traits in Yak

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